motA [2020-09-22 10:33:56]

H⁺-coupled [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|MotA]-[protein|FE56753D061344B0F10A6523F49C5C7356AA40B6|MotB] flagellar stator

locus: BSU_13690

pI: 5.01

mw: 29.19 kDa

protein length: 270 aa

gene length: 813 bp

function: [category|SW 4.1.1|Motility and chemotaxis]

product: flagellar stator subunit

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,434,433 1,435,245

Phenotypes of a mutant

• loss of swimming and swarming motility [Pubmed|24296669]
• mucoid phenotype due to the [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P activated overexpression of the ''[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]'' operon and resulting overproduction of poly-gamma-glutamate [Pubmed|24296669]
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]

The protein

Protein family

• motA family (with [protein|3D42584D65D80A1C73F057714647E29FF6BBD58A|MotP], according to UniProt)

Paralogous protein(s)

• [protein|3D42584D65D80A1C73F057714647E29FF6BBD58A|MotP]

Effectors of protein activity

• the interaction with [protein|C194336ED96FA844FFE9FB43BE59ADC5D6DDB3BE|MotI] inhibits [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|MotA] activity [Pubmed|22821967]

Structure

• [SW|6YSF] (the [protein|86C729C7D5ABEE519ADC4A893940600BBB655EF1|MotA]₅-[protein|FE56753D061344B0F10A6523F49C5C7356AA40B6|MotB]₂ complex from Clostridium sporogenes) [pubmed|32929189]

[SW|Localization]

• anchored to the cell wall, extending through the cell membrane [pubmed|29196522]

Expression and Regulation

Operons

• genes: [gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]-[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]
  description: [Pubmed|6313226]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|6313226], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

Biological materials

Mutant

• 1A631 ( ''motA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A631&Search=1A631 BGSC]
• 1A923 ( ''motA''::''erm''), [Pubmed|12813063], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A923&Search=1A923 BGSC]
• DS7498 (marker-less in NCIB3610) [Pubmed|24296669]
• BKE13690 ([gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTTTTCACCAAATCCT, downstream forward: _UP4_TTTGCAGAACAAGGAGAGGC
• BKK13690 ([gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTTTTCACCAAATCCT, downstream forward: _UP4_TTTGCAGAACAAGGAGAGGC

References

Reviews

• 31136265

Original Publications

• 26122431,6313226,21821766,22821967,24296669,24771657,28378843,29061663,29196522,32929189